human etv1 Search Results


rc210533 lenti orf  (OriGene)
90
OriGene rc210533 lenti orf
Rc210533 Lenti Orf, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+etv1/pm34624218-299-141-146?v=OriGene
Average 90 stars, based on 1 article reviews
rc210533 lenti orf - by Bioz Stars, 2026-08
90/100 stars
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etv1  (OriGene)
92
OriGene etv1
FUS is a transcriptional co-activator of PEA3 transcription factors a: Pie chart showing the distribution of FUS binding sites identified by ChIP-seq analysis in C2C12 cells. FUS binds mostly to promoters, close to the transcription start sites (TSS). b-c: Mean profiles established for FUS binding at TSS using SeqMiner in 3 independent replicates. d: Genome browser visualization of FUS binding on the promoter region of the Mrps18c and Helq genes. e: Gene ontology analysis of genes with FUS binding sites identified by ChIP-seq in C2C12 cells. f-g: MEME motif enriched in ChIP-seq peaks bound by FUS (F) and the predicted motif bound by PEA3 transcription factor ETV4 (G). h-j: Light units relative to empty control plasmid in C2C12 cells 24 h after transfection of an Mrps18c- luciferase plasmid and an expression plasmid for either hFUS WT, hFUS R495X or empty control. Cells were simultaneously transfected with si Ctr (25 nM) or si <t>ETV1</t> (25 nM, H), si ETV4 (25 nM, I) and si ETV5 (25nM, J). Nested one-way ANOVA with Tukey’s multiple comparisons test; ***P < 0.001, **P<0.01, *P < 0.05. Each dot represents the mean of an individual experiment (n=3), each consisting of 4 technical replicates. Data represent the mean ± SEM. k: Light units relative to si Ctr in C2C12 cells 24 h after transfection of Mrps18c- luciferase plasmid and si Ctr (25 nM) or si FUS and an expression plasmid for either ETV5 or empty control. Nested one-way ANOVA with Tukey’s multiple comparisons test; ***P < 0.001. Each dot represents the mean of an individual experiment (n=3), each consisting of 4 technical replicates. Data represent the mean ± SEM.
Etv1, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+etv1/bio_rxiv__2024__09__18__613669-300-0-16?v=OriGene
Average 92 stars, based on 1 article reviews
etv1 - by Bioz Stars, 2026-08
92/100 stars
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etv1 forward  (OriGene)
92
OriGene etv1 forward
FUS is a transcriptional co-activator of PEA3 transcription factors a: Pie chart showing the distribution of FUS binding sites identified by ChIP-seq analysis in C2C12 cells. FUS binds mostly to promoters, close to the transcription start sites (TSS). b-c: Mean profiles established for FUS binding at TSS using SeqMiner in 3 independent replicates. d: Genome browser visualization of FUS binding on the promoter region of the Mrps18c and Helq genes. e: Gene ontology analysis of genes with FUS binding sites identified by ChIP-seq in C2C12 cells. f-g: MEME motif enriched in ChIP-seq peaks bound by FUS (F) and the predicted motif bound by PEA3 transcription factor ETV4 (G). h-j: Light units relative to empty control plasmid in C2C12 cells 24 h after transfection of an Mrps18c- luciferase plasmid and an expression plasmid for either hFUS WT, hFUS R495X or empty control. Cells were simultaneously transfected with si Ctr (25 nM) or si <t>ETV1</t> (25 nM, H), si ETV4 (25 nM, I) and si ETV5 (25nM, J). Nested one-way ANOVA with Tukey’s multiple comparisons test; ***P < 0.001, **P<0.01, *P < 0.05. Each dot represents the mean of an individual experiment (n=3), each consisting of 4 technical replicates. Data represent the mean ± SEM. k: Light units relative to si Ctr in C2C12 cells 24 h after transfection of Mrps18c- luciferase plasmid and si Ctr (25 nM) or si FUS and an expression plasmid for either ETV5 or empty control. Nested one-way ANOVA with Tukey’s multiple comparisons test; ***P < 0.001. Each dot represents the mean of an individual experiment (n=3), each consisting of 4 technical replicates. Data represent the mean ± SEM.
Etv1 Forward, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+etv1/10__2147_slash_ott__s183435-66-7-42?v=OriGene
Average 92 stars, based on 1 article reviews
etv1 forward - by Bioz Stars, 2026-08
92/100 stars
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recombinant human etv1 protein  (Novus Biologicals)
N/A
The Recombinant Human ETV1 Protein has been validated for the following applications Western Blot ELISA Protein Array Immunoaffinity Purification
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human er81 etv1 activation kit by crispra  (OriGene)
N/A
ETV1 CRISPRa kit CRISPR gene activation of human ETS variant 1
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er81 etv1 nm 004956 human tagged orf clone  (OriGene)
N/A
Lenti ORF clone of Human ets variant 1 ETV1 transcript variant 1 Myc DDK tagged
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er81 etv1 nm 001163151 human tagged orf clone lentiviral particle  (OriGene)
N/A
Lenti ORF particles ETV1 mGFP tagged Human ets variant 1 ETV1 transcript variant 6 200ul 10 7 TU mL
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er81 etv1 nm 001163150 human tagged orf clone lentiviral particle  (OriGene)
N/A
Lenti ORF particles ETV1 mGFP tagged Human ets variant 1 ETV1 transcript variant 5 200ul 10 7 TU mL
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rabbit anti-human etv1 polyclonal antibody  (Cusabio)
N/A
Rabbit anti-Human ETV1 Polyclonal Antibody
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Image Search Results


FUS is a transcriptional co-activator of PEA3 transcription factors a: Pie chart showing the distribution of FUS binding sites identified by ChIP-seq analysis in C2C12 cells. FUS binds mostly to promoters, close to the transcription start sites (TSS). b-c: Mean profiles established for FUS binding at TSS using SeqMiner in 3 independent replicates. d: Genome browser visualization of FUS binding on the promoter region of the Mrps18c and Helq genes. e: Gene ontology analysis of genes with FUS binding sites identified by ChIP-seq in C2C12 cells. f-g: MEME motif enriched in ChIP-seq peaks bound by FUS (F) and the predicted motif bound by PEA3 transcription factor ETV4 (G). h-j: Light units relative to empty control plasmid in C2C12 cells 24 h after transfection of an Mrps18c- luciferase plasmid and an expression plasmid for either hFUS WT, hFUS R495X or empty control. Cells were simultaneously transfected with si Ctr (25 nM) or si ETV1 (25 nM, H), si ETV4 (25 nM, I) and si ETV5 (25nM, J). Nested one-way ANOVA with Tukey’s multiple comparisons test; ***P < 0.001, **P<0.01, *P < 0.05. Each dot represents the mean of an individual experiment (n=3), each consisting of 4 technical replicates. Data represent the mean ± SEM. k: Light units relative to si Ctr in C2C12 cells 24 h after transfection of Mrps18c- luciferase plasmid and si Ctr (25 nM) or si FUS and an expression plasmid for either ETV5 or empty control. Nested one-way ANOVA with Tukey’s multiple comparisons test; ***P < 0.001. Each dot represents the mean of an individual experiment (n=3), each consisting of 4 technical replicates. Data represent the mean ± SEM.

Journal: bioRxiv

Article Title: FUS controls muscle differentiation and structure through LLPS mediated recruitment of MEF2 and ETV5

doi: 10.1101/2024.09.18.613669

Figure Lengend Snippet: FUS is a transcriptional co-activator of PEA3 transcription factors a: Pie chart showing the distribution of FUS binding sites identified by ChIP-seq analysis in C2C12 cells. FUS binds mostly to promoters, close to the transcription start sites (TSS). b-c: Mean profiles established for FUS binding at TSS using SeqMiner in 3 independent replicates. d: Genome browser visualization of FUS binding on the promoter region of the Mrps18c and Helq genes. e: Gene ontology analysis of genes with FUS binding sites identified by ChIP-seq in C2C12 cells. f-g: MEME motif enriched in ChIP-seq peaks bound by FUS (F) and the predicted motif bound by PEA3 transcription factor ETV4 (G). h-j: Light units relative to empty control plasmid in C2C12 cells 24 h after transfection of an Mrps18c- luciferase plasmid and an expression plasmid for either hFUS WT, hFUS R495X or empty control. Cells were simultaneously transfected with si Ctr (25 nM) or si ETV1 (25 nM, H), si ETV4 (25 nM, I) and si ETV5 (25nM, J). Nested one-way ANOVA with Tukey’s multiple comparisons test; ***P < 0.001, **P<0.01, *P < 0.05. Each dot represents the mean of an individual experiment (n=3), each consisting of 4 technical replicates. Data represent the mean ± SEM. k: Light units relative to si Ctr in C2C12 cells 24 h after transfection of Mrps18c- luciferase plasmid and si Ctr (25 nM) or si FUS and an expression plasmid for either ETV5 or empty control. Nested one-way ANOVA with Tukey’s multiple comparisons test; ***P < 0.001. Each dot represents the mean of an individual experiment (n=3), each consisting of 4 technical replicates. Data represent the mean ± SEM.

Article Snippet: ETV1 (# TP310533), ETV4 (# TP760035), ETV5 (# TP761784) and MEF2A (# TP312830) were purchased from Origen.

Techniques: Binding Assay, ChIP-sequencing, Control, Plasmid Preparation, Transfection, Luciferase, Expressing

a: Genome browser snapshot of MEF2A, ETV5 and FUS ChIP-seq binding sites at the MRPS18C/HELQ locus. b: Venn diagram showing the overlap between FUS, MEF2A and ETV5 binding sites. Hypergeometric p-values: Fus vs Etv5: 3.66×10 -2 ; Fus vs Mef2a: <5×10 -324 ; Etv5 vs Mef2a: <5×10 -324 . c-e: Light units relative to Empty vector + si Ctr in C2C12 cells 24 h after transfection of a 3xMEF2 luciferase plasmid and an expression plasmid for either hFUS WT, hFUS R495X or empty control. Cells were simultaneously transfected with si Ctr (25 nM) or si ETV1 (25 nM, C), si ETV4 (25 nM, D), and si ETV5 (25nM, E). ETV5, but not ETV1 or ETV4, is required for co-activation of the MEF2 reporter by FUS. Nested one-way ANOVA with Tukey’s multiple comparisons test; ***P < 0.001, **P<0.01, *P < 0.05. Each dot represents the mean of an individual experiment (n=4), each consisting of 4 technical replicates. Data represent the mean ± SEM.

Journal: bioRxiv

Article Title: FUS controls muscle differentiation and structure through LLPS mediated recruitment of MEF2 and ETV5

doi: 10.1101/2024.09.18.613669

Figure Lengend Snippet: a: Genome browser snapshot of MEF2A, ETV5 and FUS ChIP-seq binding sites at the MRPS18C/HELQ locus. b: Venn diagram showing the overlap between FUS, MEF2A and ETV5 binding sites. Hypergeometric p-values: Fus vs Etv5: 3.66×10 -2 ; Fus vs Mef2a: <5×10 -324 ; Etv5 vs Mef2a: <5×10 -324 . c-e: Light units relative to Empty vector + si Ctr in C2C12 cells 24 h after transfection of a 3xMEF2 luciferase plasmid and an expression plasmid for either hFUS WT, hFUS R495X or empty control. Cells were simultaneously transfected with si Ctr (25 nM) or si ETV1 (25 nM, C), si ETV4 (25 nM, D), and si ETV5 (25nM, E). ETV5, but not ETV1 or ETV4, is required for co-activation of the MEF2 reporter by FUS. Nested one-way ANOVA with Tukey’s multiple comparisons test; ***P < 0.001, **P<0.01, *P < 0.05. Each dot represents the mean of an individual experiment (n=4), each consisting of 4 technical replicates. Data represent the mean ± SEM.

Article Snippet: ETV1 (# TP310533), ETV4 (# TP760035), ETV5 (# TP761784) and MEF2A (# TP312830) were purchased from Origen.

Techniques: ChIP-sequencing, Binding Assay, Plasmid Preparation, Transfection, Luciferase, Expressing, Control, Activation Assay